Whole Cell Patch-Clamp and SH-SY5Y tumor cells

Human neurobalstoma cells, SH-SY5Y, are widely studied and data on their electrophysiological characteristics can be found in literature. We used this cell line to set up the whole cell patch-clamp technique, not yet available in our institute, with the goal to make it suitable both for our future scientific projects and for interdisciplinary studies with other research groups. Some instruments, being outdated, have been repaired and adapted to obtain the best possible results; others were kindly borrowed by colleagues. Whole cell patch-clamp recordings were obtained and they were highly comparable with data found in literature confirming both these data and the goodness of our setup, although it needs to be updated to most recent scientific standards.